Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interferon system defects in human malignant melanoma

C Linge1, D Gewert, C Rossmann

  • 1Biology Division, Wellcome Foundation Ltd., Beckenham, Kent, United Kingdom.

Cancer Research
|September 15, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Who is Responsible? Attribution of Responsibility in the Context of Dementia: A Content-Analysis of Framing in Media Coverage.

Journal of health communication·2023
Same author

CHANGES IN GENETIC CONSTITUTION OF A POPULATION OF SPHAEROMA RUGICAUDA (CRUSTACEA: ISOPODA).

Evolution; international journal of organic evolution·2017
Same author

Squamous and Neuroendocrine Specific Immunohistochemical Markers in Head and Neck Squamous Cell Carcinoma: A Tissue Microarray Study.

Head and neck pathology·2017
Same author

Malignant salivary gland tumours of the larynx: a single institution review.

Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale·2016
Same author

Non-epithelial tumors of the larynx: a single institution review.

American journal of otolaryngology·2016
Same author

Genomic alterations in head and neck squamous cell carcinoma determined by cancer gene-targeted sequencing.

Annals of oncology : official journal of the European Society for Medical Oncology·2015
Same journal

CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor-Resistant HR+/HER2- Breast Cancer.

Cancer research·2026
Same journal

A Clinically Integrated Pediatric Patient-Derived Xenograft Program Enables Evaluation of Cohort and Patient-Specific Biology and Therapeutic Strategies.

Cancer research·2026
Same journal

Editor's Note: Heterodimerization of Insulin-like Growth Factor Receptor/Epidermal Growth Factor Receptor and Induction of Survivin Expression Counteract the Antitumor Action of Erlotinib.

Cancer research·2026
Same journal

Editor's Note: Deguelin Analogue SH-1242 Inhibits Hsp90 Activity and Exerts Potent Anticancer Efficacy with Limited Neurotoxicity.

Cancer research·2026
Same journal

Retraction: Two Functional Epitopes of Pigment Epithelial-Derived Factor Block Angiogenesis and Induce Differentiation in Prostate Cancer.

Cancer research·2026
Same journal

Editor's Note: Chronic Stress Facilitates Lung Tumorigenesis by Promoting Exocytosis of IGF2 in Lung Epithelial Cells.

Cancer research·2026
See all related articles

Melanoma cells fail to produce interferon-alpha (IFN-alpha) after viral infection, unlike normal melanocytes. This specific defect in IFN-alpha gene activation, not IFN-beta, may contribute to melanoma development.

Area of Science:

  • Immunology
  • Oncology
  • Molecular Biology

Background:

  • Interferons (IFNs) are crucial for antiviral defense.
  • Melanoma is a significant form of skin cancer.
  • The role of IFN-alpha and IFN-beta in melanoma pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate the differential secretion of IFN-alpha and IFN-beta by melanoma cells and normal melanocytes upon viral induction.
  • To identify the molecular mechanisms underlying any observed differences in IFN production.

Main Methods:

  • Virus induction of IFN-alpha and IFN-beta secretion in melanoma cell lines and normal human melanocytes.
  • Analysis of IFN-alpha mRNA translation and protein secretion.
  • Transfection studies using IFN-alpha 2b expression vectors.

Related Experiment Videos

  • Reporter construct assays to assess IFN-alpha promoter activity.

    • Main Results:

    • Normal melanocytes secreted both IFN-alpha and IFN-beta after viral induction.
    • Melanoma cells secreted IFN-beta but not IFN-alpha.
    • Melanoma cells showed normal IFN-alpha mRNA translation and protein secretion.
    • Melanoma cells exhibited defective IFN-alpha promoter activation in response to viral stimuli.
    • IFN-alpha promoter constructs were unresponsive in melanoma cells but inducible in melanocytes.

    Conclusions:

    • Melanoma cells possess a specific defect in IFN-alpha gene activation, distinct from IFN-beta.
    • This disruption is likely due to a defect in a trans-acting transcription factor required for IFN-alpha gene activation.
    • The impaired IFN-alpha response may play a role in the development and progression of malignant melanoma.