[An experimental investigation on preventive measures of skin ulcers induced by extravascular mitomycin C]

In order to make an investigation on skin ulcer induced by Mitomycin C extravasation, the preventive measures were undergone by the following ex-periment. A subcutaneous ulcerative model induced by 0.1mg Mitomycin C (MMC) extravasation in the leg of BALA/C mice was established. On the model subject, 8 pharmacologic adjuvants or physical modulation were adopted. It is preferential to choose local injection with 0.1 ml 99% dimethyl sulfoxide (DMSO), or with additional smearing DMSO on the affected skin. 10 days later, the ulcerative areas with DMSO treatment were significantly decreased in contrast with 0.1 ml 0.9% NaCI treatment (P < 0.001). The other preferential treatment were in turn by the injection of 0.1 ml 5% NaHCO3 (P < 0.001); 0.1 ml 1% procainamidi hydrochloridum (including 0.4% dexame-thasonum) (P < 0.001) or smearing with 0.1 ml 90% DMSO (including 10% Vit E) (P < 0.01). Delayed application of DMSO after MMC extravasation 30 min had been proven the limited effects on the prevention of ulcer (P < 0.05). Neither cooling of skin or local injection of sodium thiosulfate reduced MMC ulceration (P > 0.05). The mechanisms of prevention of MMC extravascular ulcer by DMSO or 5% NaHCO3 were briefly discussed.