Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

S-phase progression in synchronized human cells

¹CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, United Kingdom.

Experimental Cell Research

|September 1, 1995

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ant distribution patterns in a cameroonian cocoa plantation: investigation of the ant mosaic hypothesis.

Oecologia·2017

Same author

The spectre of 'spurious' correlations.

Oecologia·2017

Same author

Evaluation of the Pulmonary Toxicity of Ambient Particulate Matter From Camp Victory, Iraq.

Journal of toxicology and environmental health. Part A·2015

Same author

Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state.

Cell death & disease·2014

Same author

Transversal and longitudinal images from the retina of the living eye using low coherence reflectometry.

Journal of biomedical optics·2012

Same author

Optical in-fiber bragg grating sensor systems for medical applications.

Journal of biomedical optics·2012

Same journal

The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

Experimental cell research·2026

Same journal

Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

Experimental cell research·2026

Same journal

Extracellular release of cytotoxic aggregates by HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

Experimental cell research·2026

Same journal

MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

Experimental cell research·2026

Same journal

Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

Experimental cell research·2026

Same journal

Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

Experimental cell research·2026

See all related articles

DNA replication factories in human cells follow a strict S-phase program. Aphidicolin treatment reveals that each synthesis stage must complete before the next begins, highlighting dynamic nuclear organization.

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Active DNA polymerases in S-phase human cells localize to discrete replication factories.
DNA synthesis patterns correlate with replication factory appearance during S-phase progression.

Purpose of the Study:

To investigate the coordination of different phases of the DNA replication program.
To understand the dynamic nature of nuclear organization during S-phase.

Main Methods:

Synchronization of HeLa cells at the beginning of S-phase using aphidicolin.
In vitro labeling of permeabilized cells with biotin-dUTP.
Analysis of replication complexes and DNA polymerase distribution.

Main Results:

Related Experiment Videos

S-phase progression and characteristic synthesis patterns were not affected by aphidicolin treatment duration, indicating sequential phase completion.
Prolonged aphidicolin exposure did not lead to widespread origin activation.
Cells exhibited typical early S-phase patterns, with some showing reduced activity or larger, fused replication sites that reverted upon drug removal.
No biochemical defects were observed with short aphidicolin treatments; DNA polymerases resumed synthesis at in vivo rates after drug removal.

Conclusions:

The DNA replication program is tightly coordinated, with sequential completion of synthesis phases.
Nuclear organization, including replication factories, is highly dynamic.
Optimal conditions for isolating early S-phase origins of replication were established.