Related Experiment Video

Updated: Jan 23, 2026

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

702

Receptor binding of transforming growth factor-beta by human fetal adrenal cells

A K Stanković1, C R Parker

  • 1Department of Obstetrics and Gynecology, University of Alabama at Birmingham, 35233-7333, USA.

Molecular and Cellular Endocrinology
|April 1, 1995
PubMed

Insights

Adrenocorticotropic hormone (ACTH) enhances transforming growth factor beta 1 (TGF-beta 1) binding to human fetal adrenal cells. This suggests ACTH may modulate TGF-beta 1

Area of Science:

  • Endocrinology
  • Cell Biology
  • Molecular Biology

Background:

  • Transforming growth factor beta 1 (TGF-beta 1) inhibits human fetal adrenal (HFA) cell proliferation.
  • Adrenocorticotropic hormone (ACTH) partially counteracts TGF-beta 1's antimitotic effect.

Purpose of the Study:

  • To investigate if ACTH interferes with TGF-beta 1's action by altering its binding to adrenal cells.
  • To elucidate the mechanism behind ACTH's modulation of TGF-beta 1's effects.

Main Methods:

  • Incubation of HFA cells with 125I-labeled TGF-beta 1 at 4°C for varying durations.
  • Assessment of TGF-beta 1 binding inhibition by unlabeled TGF-beta 1 and other cytokines.
  • Treatment of HFA cells with ACTH, dibutyryl cAMP, or forskolin prior to TGF-beta 1 binding assays.
  • Scatchard analysis to characterize TGF-beta 1 binding sites.

Main Results:

  • TGF-beta 1 binding to HFA cells increased over time and was dose-dependently inhibited by unlabeled TGF-beta 1.
  • ACTH pretreatment significantly increased specific 125I-labeled TGF-beta 1 binding.
  • The ACTH-induced increase in TGF-beta 1 binding was maximal with 0.9 nM ACTH.
  • Dibutyryl cAMP and forskolin mimicked the effect of ACTH on TGF-beta 1 binding.
  • Scatchard analysis indicated ACTH treatment alters TGF-beta 1 binding site characteristics.

Conclusions:

  • ACTH enhances TGF-beta 1 binding to HFA cells, suggesting a novel mechanism for its modulatory role.
  • The findings imply that ACTH may influence TGF-beta 1 signaling pathways in the adrenal cortex.
  • Further research is warranted to understand the physiological implications of altered TGF-beta 1 binding in the adrenal gland.

More Related Videos

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

8.1K
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy09:16

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

Published on: September 11, 2015

8.7K

Related Concept Videos

Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
1.2K
Factors Influencing Microbial Growth: pH01:29

Factors Influencing Microbial Growth: pH

Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
1.1K
Factors Affecting Protein-Drug Binding: Drug-Related Factors01:18

Factors Affecting Protein-Drug Binding: Drug-Related Factors

Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In...
457
Factors Affecting Protein-Drug Binding: Patient-Related Factors01:29

Factors Affecting Protein-Drug Binding: Patient-Related Factors

Protein-drug binding, a pivotal aspect of pharmacokinetics, is subject to considerable variability influenced by an array of patient-related factors. The intricate interplay of age, individual differences, and pathological conditions significantly impact the binding dynamics and subsequent pharmacological effects.
Age stands as a key determinant in protein-drug binding. Neonates, characterized by low albumin content, experience heightened concentrations of unbound drugs such as phenytoin and...
310
Factors Affecting Protein-Drug Binding: Protein-Related Factors01:20

Factors Affecting Protein-Drug Binding: Protein-Related Factors

Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
534
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
3.2K