Sequence and expression of Sox-18 encoding a new HMG-box transcription factor

Area of Science:

• Genetics
• Molecular Biology
• Developmental Biology

Background:

• The Sox (Sry-like HMG-box) gene family is defined by a conserved DNA-binding domain.
• The Sry gene, a member of this family, is crucial for male sex determination in mammals.
• The existence of other Sox family members suggests broader roles in biological processes.

Purpose of the Study:

• To identify and characterize novel members of the Sox gene family.
• To determine the full cDNA sequence and protein characteristics of a newly identified Sox gene.
• To investigate the tissue-specific expression of the newly identified Sox gene in adult mice.

Main Methods:

• Gene identification and isolation techniques.
• Full cDNA sequencing to determine the complete coding sequence.
• Messenger RNA (mRNA) transcript analysis using techniques like Northern blotting or in situ hybridization (implied).

Main Results:

• Two new Sox genes, designated Sox-17 and Sox-18, were identified.
• The complete cDNA sequence of Sox-18 was determined, encoding a 378 amino acid protein.
• Sox-18 mRNA transcripts were found to be specifically expressed in adult mouse heart, lung, and skeletal muscle.

Conclusions:

• The Sox gene family is larger than previously known, with Sox-17 and Sox-18 being new additions.
• Sox-18 exhibits a distinct tissue-specific expression pattern in adult mice, suggesting roles in these tissues.
• Further research is warranted to elucidate the specific functions of Sox-18 in the heart, lung, and skeletal muscle.