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Using biopsy materials to detect mutations in gastrointestinal tumors
H Hosoi1, S Hirosawa, K Yamamoto
1First Department of Internal Medicine, Faculty of Medicine, Tokyo Medical, and Dental University, Japan.
Journal of Clinical Gastroenterology
|June 1, 1995
Summary
This study demonstrates that biopsied tumor samples are adequate for analyzing gene mutations, specifically c-K-ras and p53, aiding in carcinoma diagnosis. This approach offers a viable alternative to surgical samples for genetic analysis.
Area of Science:
- Oncology
- Molecular Biology
- Gastroenterology
Background:
- Endoscopic examinations are vital for carcinoma diagnosis.
- Analyzing gene mutations like c-K-ras and p53 can provide diagnostic insights.
- Previous studies primarily used surgical samples for gene mutation analysis.
Purpose of the Study:
- To investigate the utility of biopsied specimens for analyzing point mutations in the c-K-ras and p53 genes.
- To determine the feasibility of using only endoscopic biopsy materials for genetic mutation detection in gastrointestinal carcinomas.
Main Methods:
- Analysis of point mutations in c-K-ras and p53 genes.
- Utilized 60 colorectal and 31 gastric tumor biopsy specimens.
- Compared mutation frequencies between biopsy and surgical samples (from literature).
Main Results:
- No c-K-ras mutations were found in gastric tumors; 7 colorectal tumors showed c-K-ras mutations.
- p53 gene mutations were detected in 6 gastric carcinomas (18.2% intramucosal, 36.4% invasive).
- 8 of 15 invasive colorectal carcinomas (53.3%) exhibited p53 gene point mutations.
Conclusions:
- Biopsied specimens are adequate and useful for analyzing c-K-ras and p53 gene point mutations.
- This approach provides a valuable method for genetic analysis in gastrointestinal carcinomas using minimally invasive samples.
- Findings support the use of endoscopic biopsies for molecular diagnostics in cancer.