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Related Experiment Videos

Glutamine and alpha-ketoglutarate prevent the decrease in muscle free glutamine concentration and influence protein

B I Blomqvist1, F Hammarqvist, A von der Decken

  • 1Department of Anesthesiology and Intensive Care, Huddinge University Hospital, Sweden.

Metabolism: Clinical and Experimental
|September 1, 1995
PubMed
Summary

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Surgical trauma decreases muscle glutamine and protein synthesis. Supplementing with glutamine or alpha-ketoglutarate alone, without TPN, prevents this postoperative decline in amino acid levels and preserves protein synthesis.

Area of Science:

  • Biochemistry
  • Surgical Research
  • Metabolic Studies

Background:

  • Surgical trauma leads to decreased muscle protein synthesis and free glutamine levels.
  • Total parenteral nutrition (TPN) alone does not fully prevent these postoperative declines.
  • Glutamine or alpha-ketoglutarate supplementation with TPN can maintain muscle amino acid concentrations and protein synthesis.

Purpose of the Study:

  • To characterize total hip replacement as a human trauma model.
  • To investigate if glutamine or alpha-ketoglutarate, administered without TPN, can prevent postoperative muscle free glutamine reduction.
  • To assess the impact of these supplements on muscle protein synthesis post-surgery.

Main Methods:

  • Patients undergoing total hip replacement were randomized into control, glutamine, and alpha-ketoglutarate groups.

Related Experiment Videos

  • Participants received glucose, with or without glutamine or alpha-ketoglutarate, during surgery and for 24 hours postoperatively.
  • Muscle biopsies were analyzed for free glutamine concentration and protein synthesis markers (ribosomes, polyribosomes) before and after surgery.

    • Main Results:

    • Muscle free glutamine concentration significantly decreased in the control group but remained stable in the glutamine and alpha-ketoglutarate groups.
    • Protein synthesis, indicated by total ribosome concentration, decreased in the control group but was preserved in the supplemented groups.
    • Polyribosome concentration decreased in both control and alpha-ketoglutarate groups, suggesting differential effects.

    Conclusions:

    • Total hip replacement serves as a reproducible human trauma model.
    • Postoperative administration of glutamine or alpha-ketoglutarate alone effectively prevents the decrease in muscle free glutamine.
    • These supplements also preserve muscle protein synthesis following surgical trauma.