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Related Experiment Videos

Ultrasensitive bioanalytical assays using time-resolved fluorescence detection

E F Dickson1, A Pollak, E P Diamandis

  • 1Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario.

Pharmacology & Therapeutics
|May 1, 1995
PubMed
Summary
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Time-resolved fluorimetric detection of lanthanide chelate luminescence offers ultrasensitive bioanalytical assays. This review covers detection systems, assay formats, and applications in immunoassays and DNA hybridization.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biotechnology

Background:

  • Ultrasensitive bioanalytical assays are crucial for detecting low concentrations of biomarkers.
  • Traditional detection methods often lack the required sensitivity for certain applications.
  • Lanthanide chelate luminescence offers unique photophysical properties for enhanced detection.

Purpose of the Study:

  • To review the application of time-resolved fluorimetric detection (TRFD) of lanthanide chelate luminescence.
  • To describe assay formats, detection principles, and instrumentation for TRFD.
  • To provide an overview of TRFD in immunoassays and DNA hybridization assays.

Main Methods:

  • Review of existing literature and commercially available assays.
  • Description of the principles behind time-resolved fluorimetric detection.

Related Experiment Videos

  • Outline of detection systems, reagents, and instrumentation used in TRFD.

    • Main Results:

    • TRFD of lanthanide chelate luminescence enables ultrasensitive detection in bioassays.
    • Various assay formats, including immunoassays and DNA hybridization assays, benefit from this technique.
    • Detailed description of necessary components for implementing TRFD systems.

    Conclusions:

    • Time-resolved fluorimetric detection of lanthanide chelate luminescence is a powerful tool for ultrasensitive bioanalysis.
    • The technique is well-established and commercially available for various applications.
    • Further development can expand its utility in complex biological samples.