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Ultrasensitive bioanalytical assays using time-resolved fluorescence detection
E F Dickson1, A Pollak, E P Diamandis
1Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario.
Pharmacology & Therapeutics
|May 1, 1995
Abstract:
This article reviews the use of time-resolved fluorimetric detection of lanthanide chelate luminescence as a detection method for ultrasensitive bioanalytical assays. Assay formats and detection methods, and the principle of time-resolved fluorimetric detection, are described. Detection systems, assay formats, reagents, and instrumentation for time-resolved fluorimetric detection are outlined. A review of published and commercially available immunoassays and DNA hybridization assays using time-resolved fluorimetric detection of lanthanide chelate luminescence is given.