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Effects of gypenosides on mouse splenic lymphocyte transformation and DNA polymerase II activity in vitro
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|July 1, 1995
Abstract
Aim:
To study the effects of gypenosides (Gyp) on lymphocyte transformation and DNA polymerase II activity.
Methods:
Lymphocyte transformation response was induced by concanavalin A and lipopolysaccharides respectively. The activity of DNA polymerase II and DNA synthesis were assayed with TTP and [3H]TdR incorporation respectively in mixed lymphocyte culture test.
Results:
Gyp 2.5-20 mg L-1 enhanced splenic T- and B- cell transformation, increased the DNA synthesis and potentiated the activity of DNA polymerase II. However, Gyp > 40 mg L-1 showed contrary effects.
Conclusion:
Gyp regulated lymphocyte transformation and DNA synthesis by regulating DNA polymerase II activity.