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Updated: Jul 11, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
cDNA cloning of human cytosolic platelet-activating factor acetylhydrolase gamma-subunit and its mRNA expression in
H Adachi1, M Tsujimoto, M Hattori
1Suntory Institute for Biomedical Research, Osaka, Japan.
Abstract:
The cDNA for human cytosolic platelet-activating factor acetylhydrolase (PAF-AH) gamma-subunit was isolated and the gene structure was elucidated. The complete amino acid sequence deduced from cDNA contains 231 amino acid residues. Southern blot analysis indicated the presence of a single gene. Northern blot analysis showed that 1.0 kb transcript was expressed in a tissue specific manner. Comparison of the expression pattern between adult and fetal tissues suggests that the enzyme plays an important role during the development of brain.

