Acylated flavonol sophorotriosides from pea shoots

Seven flavonols were isolated and identified from Pisum sativum (cv Solara) shoots. The 3-glucoside, and 3-sophorotrioside [beta-D-glucopyranosyl(1-->2)-beta-D- glucopyranosyl(1-->2)-beta-D-glucopyranoside] of quercetin, the 3-sophorotrioside of kaempferol, and the acylated derivatives of quercetin 3-sophorotrioside with p-coumaric, caffeic, ferulic and sinapic acids on the hydroxyl at the 6-position of the terminal sugar. The caffeic and sinapic acid esters are two new naturally occurring compounds. This is the first report in which the structures of the p-coumaryl- and ferulyl-sophorotriosides of quercetin, which were previously reported from pea leaves, have been completely established by means of 1H NMR studies including COSY, NOESY and TOCSY experiments.