New lipid mixture for efficient lipid-mediated transfection of BHK cells

A crude fraction of soybean lipids (azolectin) proved to be an inexpensive and effective substitute for highly purified phosphatidylethanolamine in preparation of phospholipid/dimethyldioctadecyl-ammonium bromide liposomes. Furthermore, directly suspending the components in aqueous buffer was superior to first dissolving the lipids in CHCl3.