Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ternary complex formation on leaderless phage mRNA

A Resch1, K Tedin, A Graschopf

  • 1Institute of Microbiology and Genetics, University of Vienna, Austria.

FEMS Microbiology Reviews
|August 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Different flames, different fates : A comparative study of surgical interventions and critical care burden in electrical vs. thermal burn injuries.

Wiener klinische Wochenschrift·2026
Same author

Phenotypic zinc resistance does not correlate with antimicrobial multi-resistance in fecal <i>E. coli</i> isolates of piglets.

Gut pathogens·2020
Same author

Modification of translation factor aIF5A from Sulfolobus solfataricus.

Extremophiles : life under extreme conditions·2018
Same author

Cross-talk Between Host, Microbiome and Probiotics: A Systems Biology Approach for Analyzing the Effects of Probiotic Enterococcus faecium NCIMB 10415 in Piglets.

Molecular informatics·2016
Same author

[Not Available].

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2016
Same author

[Nucleic structure in relation to functional change in the stomata of Vicia faba].

Planta·2014
Same journal

Feeding the invaders: How metabolic imbalance shapes infection and biofilm development.

FEMS microbiology reviews·2026
Same journal

Interactions between extracellular vesicles and viruses: lessons learned across species and kingdoms.

FEMS microbiology reviews·2026
Same journal

Killer Peptide: an antibody-derived self-assembling peptide bridging antimicrobial and host-defense mechanisms.

FEMS microbiology reviews·2026
Same journal

From gatekeeper to target: MAPK cascades as control circuits at the insect-microbe interface.

FEMS microbiology reviews·2026
Same journal

The role of fungal G protein-coupled receptors in interspecies cell-cell communication.

FEMS microbiology reviews·2026
Same journal

Convergent symbioses: morphology, life history, and niche specialization in coral and lichen mutualisms.

FEMS microbiology reviews·2026
See all related articles

Investigating phage mRNA translation, this study found that altering downstream sequences did not significantly affect gene expression. Ribosomal protein S1 may play a role in recognizing the cI mRNA.

Area of Science:

  • Molecular Biology
  • Microbial Genetics
  • Gene Expression Regulation

Background:

  • Bacterial transcription and translation initiation are crucial for gene expression.
  • Bacteriophages utilize specific mRNA structures and host factors for efficient protein synthesis.
  • The translational efficiency of bacteriophage mRNAs is influenced by sequences surrounding the start codon.

Purpose of the Study:

  • To investigate the role of downstream sequences in the translational efficiency of bacteriophage Lambda (λ) cI mRNA and phage P2 gene V mRNA.
  • To determine the impact of mutations, including deletions of the putative downstream box, on mRNA expression.
  • To explore the potential involvement of ribosomal protein S1 in mRNA recognition.

Main Methods:

  • Utilized lacZ (β-galactosidase) fusion analysis to assess translational efficiency.

Related Experiment Videos

  • Introduced mutations, including deletions, in the immediate downstream coding regions of cI and gene V mRNAs.
  • Employed primer extension inhibition analysis to study mRNA-ribosome interactions.

    • Main Results:

    • Alterations in the immediate downstream coding region, including deletions of the putative downstream box, did not significantly reduce the expression of lacZ fusions for either cI or gene V mRNAs.
    • Primer extension inhibition analysis indicated a potential role for ribosomal protein S1 in the recognition of cI mRNA.

    Conclusions:

    • The downstream box is not essential for efficient translation initiation of cI and gene V mRNAs under the studied conditions.
    • Ribosomal protein S1 might be involved in the specific recognition of bacteriophage Lambda cI mRNA, suggesting a role beyond general translation initiation.
    • Further research is warranted to elucidate the precise mechanisms of phage mRNA translational control and the involvement of specific ribosomal proteins.