Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BCL6 encodes a sequence-specific DNA-binding protein

B W Baron1, R R Stanger, E Hume

  • 1Department of Pathology, University of Chicago, IL 60637, USA.

Genes, Chromosomes & Cancer
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of relativistic laser intensity on isochoric heating of metal wire targets.

Optics express·2021
Same author

Cell therapy for posttraumatic shoulder osteonecrosis.

Morphologie : bulletin de l'Association des anatomistes·2020
Same author

Deep learning for screening of interstitial lung disease patterns in high-resolution CT images.

Clinical radiology·2020
Same author

Four-year follow-up of two patients on maintenance therapy with fondaparinux and mycophenolate mofetil for microthrombotic antiphospholipid syndrome.

Lupus·2019
Same author

'Hot Joints' infection protocol reduces unnecessary post-operative re-admissions following total hip and knee arthroplasty.

The bone & joint journal·2017
Same author

Return to sports and quality of life after high tibial osteotomy in patients under 60 years of age.

Orthopaedics & traumatology, surgery & research : OTSR·2017
Same journal

Biphenotypic Sinonasal Sarcoma With a Novel PAX3::MAML2 Fusion.

Genes, chromosomes & cancer·2026
Same journal

An Affordable and Efficient In-House Approach for Detecting Gene Fusions in Bone and Soft Tissue Tumors Using a Custom Capture Panel and Nanopore Sequencing.

Genes, chromosomes & cancer·2026
Same journal

PKMYT1 in Cancer: Beyond Cell Cycle Checkpoints to Context-Dependent Therapeutic Vulnerability.

Genes, chromosomes & cancer·2026
Same journal

Associations of Dietary Intake Traits and Aging Indicators With Colorectal Cancer: A Mendelian Randomization Study.

Genes, chromosomes & cancer·2026
Same journal

Primary Tumor-Associated Loss of the Y Chromosome and Clinical Outcome in Metastatic Colorectal Cancer.

Genes, chromosomes & cancer·2026
Same journal

Cytogenetic and Molecular Analysis of a "Double-Hit" RUNX1 Including a RUNX1 p.Trp279* and a Cryptic Novel t(6;21)(q25;q22)/RUNX1::ARID1B in Acute Myeloid Leukemia.

Genes, chromosomes & cancer·2026
See all related articles

Researchers identified the specific DNA sequence recognized by the BCL6 zinc finger region using cyclic amplification and selection of targets (CASTing). This finding advances understanding of BCL6

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Chromosomal rearrangements involving the BCL6 gene are frequently observed in diffuse large-cell lymphomas.
  • Understanding the DNA-binding specificities of key proteins like BCL6 is crucial for deciphering oncogenic mechanisms.

Purpose of the Study:

  • To determine the precise DNA-binding site of a glutathione-S-transferase fusion protein containing the BCL6 zinc finger region.
  • To characterize the DNA sequence recognized by the BCL6 protein.

Main Methods:

  • Cyclic amplification and selection of targets (CASTing) was employed to identify DNA sequences bound by the BCL6 protein.
  • Oligonucleotides with random central bases were incubated with the protein, and binding sequences were amplified via polymerase chain reaction (PCR).

Related Experiment Videos

  • Binding sequences were cloned, sequenced, and analyzed to establish a consensus binding motif.

    • Main Results:

    • A consensus DNA-binding sequence, TTTNNNGNNATNCTTT, was identified for the BCL6 zinc finger region.
    • Mutational analysis confirmed the specificity of the 3' CTTT motif within the consensus sequence.
    • Constant base pairs in the CASTing oligonucleotides were also found to contribute to protein binding.

    Conclusions:

    • The study successfully elucidated the DNA-binding preference of the BCL6 zinc finger region.
    • The identified consensus sequence provides a foundation for further research into BCL6 transcriptional regulation in lymphomas.
    • These findings contribute to a deeper understanding of the molecular basis of BCL6-associated malignancies.