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Related Experiment Videos

Ascorbic acid during cerebral ischemia in newborn piglets

D Oriot1, K Beharry, J B Gordon

  • 1Neonatal Research, Montreal Children's Hospital, McGill University, Quebec, Canada.

Acta Paediatrica (Oslo, Norway : 1992)
|June 1, 1995
PubMed
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Newborn piglets experiencing cerebral ischemia showed a significant drop in brain ascorbic acid. However, cerebrospinal fluid (CSF) ascorbic acid levels rose during reperfusion, suggesting brain release during ischemia.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Perinatal Medicine

Background:

  • Cerebral ischemia in newborns is a critical condition affecting brain development.
  • Ascorbic acid (vitamin C) plays a vital role in brain function and antioxidant defense.
  • Understanding ascorbic acid dynamics during ischemic events is crucial for therapeutic strategies.

Purpose of the Study:

  • To investigate the changes in ascorbic acid levels in the brain, cerebrospinal fluid (CSF), and blood during and after experimental cerebral ischemia in newborn piglets.
  • To determine if cerebral ischemia leads to significant oxidation of ascorbic acid within brain tissue.

Main Methods:

  • Newborn piglets underwent bilateral carotid artery ligation to induce cerebral ischemia, followed by a reperfusion period.
  • Ascorbic acid (reduced and oxidized forms) concentrations were measured in brain tissue, CSF, and blood samples collected before, during, and after the ischemic event.

Related Experiment Videos

  • Cerebral blood flow and cerebral metabolic rate of oxygen were monitored to confirm the ischemic state.

    • Main Results:

    • Bilateral carotid ligation significantly reduced cerebral blood flow by 54% and cerebral metabolic rate of oxygen by 43%.
    • Total brain ascorbic acid content decreased by 60% after ischemia and reperfusion.
    • CSF ascorbic acid levels increased significantly during reperfusion, rising by 60% at 30 minutes and 160% at 120 minutes, while blood ascorbic acid remained unchanged.

    Conclusions:

    • Cerebral ischemia in newborn piglets leads to a substantial decrease in brain ascorbic acid.
    • The observed increase in CSF ascorbic acid during reperfusion, coupled with minimal brain oxidation, suggests that the brain releases ascorbic acid during ischemic periods.
    • These findings highlight a potential endogenous protective mechanism involving ascorbic acid release from the brain during ischemia.