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Using Fyrite to monitor incubator carbon dioxide levels
J E Johnson1, W R Boone, S T Lee
1Greenville Hospital System, Reproductive Endocrinology Associates, South Carolina 29603, USA.
Purpose:
The Fyrite analyzer (Bacharach, Incorporated) was evaluated to ascertain whether it would perform within the range specified by the manufacturer (+/- 0.5%). Fyrite analyzer samples were compared to control samples obtained using an infrared CO2 monitor (Ohmeda, Model Number 5200).
Methods:
Ten Fyrite analyzer measurements from each of three incubators were obtained using two different analyzers by three investigators for a total of 60 measurements per investigator. Twenty control measurements for each of the three incubators were obtained using an infrared monitor calibrated at the 5.0% CO2 level.
Results:
Fyrite analyzer values fell outside of an acceptable range (Control mean CO2 +/- 0.5%) for 7 of 60 (11.7%) observations for Incubator 1 and 4 of 60 (6.7%) observations for Incubator 2. (Incubator 3 had no values outside the range.) The Fyrite analyzers were significantly different from each other (P = 0.002). The three investigators (I) differed significantly from the control (I1 and I3, P < 0.001; I2, P = 0.02) as well as from each other (I1 vs I2, P < 0.001; I1 vs I3, P < 0.001; I2 vs I3, P < 0.016).
Conclusions:
Use of an infrared monitor for incubator calibration and monthly CO2 checks in concert with daily use of the Fyrite as a monitoring tool only is the better method.