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Endothelial cells adherent to expanded polytetrafluoroethylene express the intercellular adhesion molecule-1
M S Margiotta1, L Benton, R S Greco
1Department of Surgery, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903-0019, USA.
Journal of the American College of Surgeons
|September 1, 1995
Summary
Expanded polytetrafluoroethylene (ePTFE) surfaces increase intercellular adhesion molecule-1 (ICAM-1) antibody binding on endothelial cells. This biomaterial activation is a key part of the inflammatory response to implants.
Area of Science:
- Biomaterials Science
- Immunology
- Cell Biology
Background:
- The foreign body reaction to biomaterials is not fully understood.
- Expanded polytetrafluoroethylene (ePTFE) is a common implant material.
- Intercellular adhesion molecule-1 (ICAM-1) plays a role in inflammatory responses.
Purpose of the Study:
- To investigate the effect of ePTFE surfaces on endothelial cell binding of ICAM-1 antibodies.
- To evaluate ePTFE's role in the inflammatory response of implanted biomaterials.
Main Methods:
- Human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC) were cultured on ePTFE and polystyrene.
- Cells were analyzed for ICAM-1 antibody binding.
- Leukocytes (PBLs) were cocultured with HUVEC on ePTFE and polystyrene.
Main Results:
- Endothelial cell adherence to ePTFE increased ICAM-1 antibody binding twofold compared to controls.
- Coculture with PBLs further increased ICAM-1 antibody binding significantly on both ePTFE and polystyrene.
Conclusions:
- Endothelial cell adherence to ePTFE activates cells, increasing ICAM-1 antibody binding.
- This activation is a component of the multicellular inflammatory response to biomaterials.
- ePTFE surfaces are associated with cellular activation relevant to implant integration.