A compared TEM/SEM investigation on the pericytic investment in developing microvasculature of the chick optic tectum

The pericytes have been ultrastructurally and morphometrically analyzed in the neural vessels of the chick embryo optic tectum, under the transmission and scanning electron microscopes. The observations demonstrated that shape and surface features of pericytes, as well as their spatial relation with the endothelium, remarkably change during development, whereas their ultrastructure does not substantially modify from the early to the late embryonic stages. The pericytes have an ovoid body, broad processes, smooth surfaces, and are closely applied to the endothelial tube on days 5-7; they show convolute shape, highly irregular surfaces, and are complicately interdigitated with the endothelial cells, when a vivacious vessel growth takes place, on days 12-14; finally, they are flattened, smooth, highly branched, and completely enclosed in the basement lamina on days 20-21, when a definitive vascular pattern is established. The contribution of pericytes to the formation of the basement lamina has been confirmed by the detection of exocytotic vesicles discharging their content toward the subendothelial cleft. The morphometric evaluations revealed that pericytes provide the growing neural microvessels with an almost continuous coverage which, however, undergoes a significant reduction within hatching time. On the whole, the results suggest that the pericytes are as active as the endothelial cells during the vessel growth and play an inhibitory role on the endothelial proliferation only later on, when they are closely adherent to the endothelium and are encompassed by the basement lamina.