Related Experiment Video
Updated: Aug 8, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
Roles of the phosphorylation of human interleukin 1 alpha in proteolytic processing
1Department of Biomolecular Science, Faculty of Science, Toho University, Chiba, Japan.
Abstract:
To investigate the role of phosphorylation of a precursor form of interleukin-1 alpha (pre-IL-1 alpha), we obtained cells producing either phosphorylated or unphosphorylated pre-IL-1 alpha. Although calcium-dependent proteolytic processing of unphosphorylated pre-IL-1 alpha could be observed in cell lysates, proteolytic processing was not induced by treatment with calcium ionophore in intact cells producing the unphosphorylated pre-IL-1 alpha. Further, unphosphorylated pre-IL-1 alpha showed no calcium-dependent binding to acidic phospholipids at concentrations below 5 x 10(-6) M. These results suggested that phosphorylated pre-IL-1 alpha became susceptible to proteolytic processing by association with the cell membrane in a calcium-dependent manner.
Related Concept Videos
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Protein Kinases and Phosphatases
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Amplifying Signals via Enzymatic Cascade
The JAK-STAT Signaling Pathway
IP3/DAG Signaling Pathway

