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Published on: January 25, 2018
Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean
P J Maughan1, M A Saghi Maroof, G R Buss
1Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.
Genome
|August 1, 1995
Summary
Microsatellites (SSRs) reveal extensive genetic variation in soybean, with wild soybean exhibiting greater allelic diversity than cultivated varieties. Amplified sequence length polymorphisms (ASLPs) also show utility as genetic markers.
Area of Science:
- Plant genetics
- Molecular biology
- Agricultural science
Background:
- Genetic markers are crucial for understanding crop diversity and facilitating breeding.
- Soybean (Glycine max and Glycine soja) genetic resources require robust marker systems for characterization.
Purpose of the Study:
- To quantify genetic variation in soybean using microsatellites (SSRs) and amplified sequence length polymorphisms (ASLPs).
- To evaluate the efficacy of SSRs and ASLPs as genetic markers for soybean.
- To compare allelic diversity between wild and cultivated soybean accessions.
Main Methods:
- Analysis of genetic variation using five SSR loci across 94 soybean accessions.
- Segregation analysis of SSRs in F2 populations to identify loci and linkage groups.
- Assay of three ASLP loci to determine allelic diversity.
Main Results:
- SSR analysis detected 79 alleles across five loci, with 5 to 21 alleles per locus, representing high allelic diversity.
- Wild soybean (Glycine soja) displayed significantly greater allelic diversity for SSR loci compared to cultivated soybean (Glycine max).
- ASLP analysis identified two alleles per locus, with higher diversity observed in cultivated soybean.
Conclusions:
- SSRs are highly effective genetic markers for soybean, particularly for species with low variation detected by other methods.
- ASLPs offer potential as genetic markers for site-directed mapping in soybean.
- The findings highlight the importance of wild soybean as a source of genetic diversity for crop improvement.
