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Related Experiment Videos

Proton-activated currents in chick spinal motoneurons

H Hatt1, J L Rosenheimer, D O Smith

  • 1Physiologisches Institut der Technischen Universität München, Germany.

Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology
|October 1, 1995
PubMed
Summary

Transient decreases in extracellular pH activate large inward currents in embryonic chick motoneurons. These proton-activated currents inactivate rapidly and reactivate slowly, potentially impacting neuronal function during pathological conditions.

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Area of Science:

  • Neuroscience
  • Electrophysiology
  • Ion Channel Physiology

Background:

  • Extracellular pH fluctuations can influence neuronal excitability.
  • Proton-activated channels play a role in neuronal signaling.

Purpose of the Study:

  • To characterize proton-activated currents in embryonic chick motoneurons.
  • To investigate the properties of these currents, including activation, inactivation, and ion selectivity.

Main Methods:

  • Patch-clamp recordings from embryonic chick motoneurons.
  • Rapid application of protons to alter extracellular pH.
  • Single-channel recordings to analyze channel kinetics and conductance.

Main Results:

  • Proton application evoked large, inactivating inward currents.

Related Experiment Videos

  • Half-maximal activation (EC50) occurred at pH 6.8.
  • Single-channel analysis revealed fast activation, specific open/closed times, and a conductance of 22 pS at pH 6.5.
  • Currents were modulated by Ni2+ and Ca2+ concentrations.
  • Inactivation was pH-dependent but not voltage-dependent, with slow reactivation.
  • Proton- and glutamate-activated currents showed summation.

    • Conclusions:

    • Transient extracellular acidity activates inward currents in motoneurons.
    • These currents exhibit rapid inactivation and slow reactivation.
    • Such currents may be relevant in pathological states affecting extracellular pH.