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[Haemophilus isolates in children suffering from chronic upper respiratory tract infections]
J Chylak1, Z Cybulski, T Tułecka
1Zakład Mikrobiologii Lekarskiej, Instytut Mikrobiologii i Chorób Zakaźnych A.M., Poznaniu.
Abstract:
For a three year period, between 1992 and 1994, 271 isolates of H. influenzae and H. parainfluenzae were examined using biochemical tests, beta-lactamase production and antibiotic susceptibility patterns. H. influenzae was examined by serological typing using polyvalent and monovalent sera a-f (Difco), as well. The strains were derived from children who were treated because of chronic pharyngitis. Sensitivity tests to Erythromycin, Gentamycin, Cefradine, Ampicillin, Doxycycline, Cefuroxime, Amoxicillin/Clavulanic Acid, Co-Trimoxazole, were performed with a standard disk-diffusion method. Biotyping was performed using API NH set (bioMerieux) and the ability to produce beta-lactamase was measured using iodometric method. Of 157 H. influenzae, 58 (36.9%) were typeable with H. influenzae antisera. The most common biotypes of these strains were I and II although biotypes III-VII were presented, as well. Of 114 H. parainfluenzae most of the strains showed the biotypes I and II. Its worth noting that 14% of H. influenzae and 15.8% of H. parainfluenzae strains were capable of beta-lactamase production. The strains of H. influenzae and H. parainfluenzae were most resistant to Cefradine and Co-Trimoxazole, respectively.
Insights
This study examined antibiotic resistance in Haemophilus influenzae and Haemophilus parainfluenzae strains from children with chronic pharyngitis. A significant percentage of strains showed resistance to common antibiotics, highlighting potential treatment challenges.
Area of Science:
- Medical Microbiology
- Bacteriology
- Antimicrobial Resistance
Background:
- Haemophilus influenzae and Haemophilus parainfluenzae are significant human pathogens.
- Chronic pharyngitis in children can be associated with these bacterial infections.
- Understanding the antimicrobial susceptibility patterns of these bacteria is crucial for effective treatment.
Purpose of the Study:
- To characterize the biotypes and serotypes of H. influenzae and H. parainfluenzae isolates.
- To determine the prevalence of beta-lactamase production in these strains.
- To assess the antibiotic susceptibility patterns of H. influenzae and H. parainfluenzae against various antimicrobial agents.
Main Methods:
- Biochemical tests and biotyping using API NH set.
- Serological typing of H. influenzae using polyvalent and monovalent antisera.
- Antibiotic susceptibility testing via disk-diffusion method and beta-lactamase production measurement using iodometric method.
Main Results:
- Of 157 H. influenzae isolates, 36.9% were typeable, with biotypes I and II being most common.
- 14% of H. influenzae and 15.8% of H. parainfluenzae strains produced beta-lactamase.
- H. influenzae showed highest resistance to Cefradine, while H. parainfluenzae was most resistant to Co-Trimoxazole.
Conclusions:
- The study identified common biotypes and serotypes for H. influenzae and H. parainfluenzae in pediatric chronic pharyngitis cases.
- Beta-lactamase production was observed in a notable proportion of isolates.
- Emerging antibiotic resistance patterns necessitate careful consideration in clinical management of infections caused by these bacteria.