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Related Experiment Videos

Complementable and noncomplementable host adaptation defects in bipartite geminiviruses

I T Petty1, C G Miller, T J Meade-Hash

  • 1Department of Microbiology, North Carolina State University, Raleigh 27695-7615, USA.

Virology
|September 10, 1995
PubMed
Summary
This summary is machine-generated.

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Tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV) show distinct host ranges due to poor virus-host interactions. TGMV has a defect in beans, while BGMV has low DNA accumulation in N. benthamiana.

Area of Science:

  • Plant virology
  • Molecular biology
  • Genetics

Background:

  • Geminiviruses, including tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV), infect diverse plant species.
  • Despite genetic similarity, individual geminiviruses exhibit distinct host ranges, with common hosts not previously identified for TGMV and BGMV.

Purpose of the Study:

  • To investigate the genetic basis for the distinct host ranges of TGMV and BGMV.
  • To identify specific viral components and interactions responsible for host adaptation or limitation.

Main Methods:

  • Introduction of cloned viral genome components (A and B) of TGMV and BGMV into Nicotiana benthamiana and bean (Phaseolus vulgaris).
  • Assessing viral infectivity, virulence, and DNA accumulation in different host plants.

Related Experiment Videos

  • Complementation experiments involving co-inoculation with different viral components to identify defective functions.

    • Main Results:

    • TGMV demonstrated low specific infectivity and virulence in bean plants, indicating a noncomplementable host adaptation defect.
    • BGMV showed high specific infectivity in N. benthamiana, but infections were asymptomatic with low viral DNA accumulation.
    • The BGMV phenotype in N. benthamiana was partially complemented by TGMV A component and fully by the complete TGMV genome, suggesting a virus-nonspecific trans-acting factor defect.

    Conclusions:

    • Host range limitation in bipartite geminiviruses can result from multiple, distinct defective virus-host interactions.
    • TGMV's defect in bean appears to be cis-acting or virus-specific trans-acting.
    • BGMV's poor adaptation to N. benthamiana involves a virus-nonspecific trans-acting factor encoded by its A component.