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Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
Published on: September 15, 2010
[Stability and virus-inhibiting effect of recombinant receptor toxins based on the human CD4 receptor and diphtheria
Abstract:
The causes of differences in the stability of CD4 receptor and diphtherial toxin based recombinant receptorotoxins synthesized in E.coli and differing by their primary structure were under study. Insertion of a CD4 receptor fragment, responsible for HIV binding, on the N terminus of hybrid protein was found to lead to a drastic reduction of the stability of the hybrid polypeptide in E.coli and to impossibility of obtaining full-size protein product. In vitro experiments on a model of human T lymphocyte culture demonstrated that recombinant receptorotoxin stably expressed in E.coli inhibited the cytopathic effect of type 1 HIV and the effect of syncytium formation induced by the virus.
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