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Optimization of radioligand binding assays for I1-imidazoline sites

P Ernsberger1, J E Piletz, L M Graff

  • 1Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio 44106-4982, USA.

Annals of the New York Academy of Sciences
|July 12, 1995
PubMed
Abstract

No abstract available in PubMed .

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:

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