Related Experiment Video
Updated: Jul 23, 2026

08:40
An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
Published on: March 14, 2016
Optimization of radioligand binding assays for I1-imidazoline sites
P Ernsberger1, J E Piletz, L M Graff
1Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio 44106-4982, USA.
Annals of the New York Academy of Sciences
|July 12, 1995
Abstract
No abstract available in PubMed .
Related Concept Videos
Ligand Binding Sites
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
The Equilibrium Binding Constant and Binding Strength
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:

