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Updated: Aug 17, 2026

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
Published on: November 26, 2013
17-beta-Estradiol stimulation of endothelial K+ channels
1Department of Pharmacology & Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Abstract:
Whole-cell membrane current recordings under voltage clamp were made from freshly isolated rabbit aortic endothelial cells. Intracellular Ca2+ signals of fura-2 loaded both native single aortic endothelial cells and intact endothelium from rabbit cardiac valves were measured using imaging fluorescence microscopy. Application of 17-beta-estradiol stimulated rapid discharge of transient outward currents and induced an increase in the intracellular free Ca2+ concentration ([Ca2+]i. The stimulated discharge of outward currents and [Ca2+]i were reduced by extracellulary applied tetraethylammonium (TEA).
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