Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of basic proteins from goldfish myelin

F G Mastronardi1, C Boulias, B I Roots

  • 1Department of Zoology, University of Toronto, Ontario, Canada.

Journal of Neurochemistry
|January 1, 1993
PubMed
Summary

Goldfish myelin basic protein (MBP) shares epitopes with mammalian MBP but lacks microheterogeneity. Goldfish MBP is less cationic and has higher serine content than mammalian and shark MBPs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perspectives on participation of a person with communication disabilities in a rehabilitation research study.

European journal of physical and rehabilitation medicine·2014
Same author

Acyl and alkenyl group composition of brain subcellular fractions of goldfish (Carassius auratus L.) acclimated to different environmental temperatures.

Neurochemical research·2013
Same author

NMDA receptor encephalitis mimicking seronegative neuromyelitis optica.

Neurology·2010
Same author

Interdigitation of Fatty Acid chains of dipalmitoylphosphatidylglycerol due to intercalation of myelin basic protein.

Biophysical journal·2009
Same author

Synergy between paclitaxel plus an exogenous methyl donor in the suppression of murine demyelinating diseases.

Multiple sclerosis (Houndmills, Basingstoke, England)·2007
Same author

Multiple sclerosis: an important role for post-translational modifications of myelin basic protein in pathogenesis.

Molecular & cellular proteomics : MCP·2003

Area of Science:

  • Neuroscience
  • Biochemistry
  • Comparative Biology

Background:

  • Myelin basic protein (MBP) is a key component of myelin sheath.
  • Understanding MBP evolution and variations across species provides insights into myelin structure and function.

Purpose of the Study:

  • To characterize the myelin basic protein (MBP) from the common goldfish (Carassius auratus).
  • To compare goldfish MBP with mammalian and other vertebrate MBPs.

Main Methods:

  • Immunological cross-reactivity assays using polyclonal and monoclonal antibodies.
  • Protein iodination and carboxymethyl cellulose chromatography for charge heterogeneity analysis.
  • Urea alkaline gel electrophoresis and reversed-phase High-Performance Liquid Chromatography (HPLC) for isoform separation.

Related Experiment Videos

  • Amino acid composition analysis and partial sequence analysis via chemical cleavage.

    • Main Results:

    • Goldfish MBP exhibited immunological cross-reactivity with antibodies against bovine MBP and a conserved mammalian epitope.
    • Goldfish MBP was less cationic than human MBP and lacked the microheterogeneity observed in higher vertebrates.
    • Amino acid analysis revealed similarities to other MBPs, but with a higher serine content.
    • HPLC separated goldfish MBP into 17- and 14-kDa isoforms.

    Conclusions:

    • Goldfish MBP shares conserved epitopes with mammalian MBP, indicating evolutionary conservation.
    • The lack of microheterogeneity in goldfish MBP suggests divergence in post-translational modifications or protein structure compared to higher vertebrates.
    • Higher serine content in goldfish MBP may indicate unique functional or structural roles.