Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biospecific interaction analysis using biosensor technology

M Malmqvist1

  • 1Pharmacia Biosensor AB, Uppsala, Sweden.

Nature
|January 14, 1993
PubMed
Summary

A novel biosensor system analyzes protein interactions in solution without labels. This technology reveals key characteristics like kinetics and binding position for enhanced understanding of biospecific interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.

Scientific reports·2017
Same author

Predicting the kinetics of peptide-antibody interactions using a multivariate experimental design of sequence and chemical space.

Journal of molecular recognition : JMR·2001
Same author

Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space.

Proteins·1999
Same author

Initiation of Escherichia coli ribosomes on matrix coupled mRNAs studied by optical biosensor technique.

Biochimie·1999
Same author

Identification and optimization of regeneration conditions for affinity-based biosensor assays. A multivariate cocktail approach.

Analytical chemistry·1999
Same author

BIACORE: an affinity biosensor system for characterization of biomolecular interactions.

Biochemical Society transactions·1999

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Understanding biospecific interactions is crucial in various scientific fields.
  • Current analytical methods may have limitations in characterizing these interactions comprehensively.

Purpose of the Study:

  • To introduce and validate a new analytical system for studying biospecific interactions.
  • To examine functional characteristics such as kinetics, affinity, and binding position.

Main Methods:

  • Utilizing biosensor technology for label-free analysis.
  • Analyzing proteins in free solution binding to an immobilized ligand.
  • Employing a hydrophilic sensor surface for interaction studies.

Main Results:

  • The system provides fundamental information on biospecific interactions.
  • Label-free analysis successfully characterized kinetics, affinity, and binding position.
  • The hydrophilic sensor surface facilitated effective protein binding.

Conclusions:

  • The developed biosensor system offers a powerful tool for studying biospecific interactions.
  • Label-free analysis in solution provides valuable insights into interaction dynamics.
  • This technology enhances the understanding of molecular recognition events.

Related Experiment Videos