Related Experiment Videos
F1Fo-ATPase subunit e gene isolated in a screen for diet regulated genes
T S Elliott1, D A Swartz, E A Paisley
1Department of Internal Medicine, University of Illinois College of Medicine, Urbana.
Biochemical and Biophysical Research Communications
|January 15, 1993
Summary
Researchers developed a new protocol to find genes affected by diet. They discovered LFM-1 (Low Fat Mammary), a gene expressed differently in mice on low-fat diets, particularly in mammary glands.
Area of Science:
- Molecular Biology
- Nutritional Science
- Gene Expression Analysis
Background:
- Dietary nutrient levels significantly influence gene regulation.
- Understanding nutrient-regulated genes is crucial for metabolic and disease research.
- Mammary gland gene expression is sensitive to dietary fat.
Purpose of the Study:
- To establish a protocol for isolating genes differentially regulated by dietary nutrient levels.
- To identify novel genes involved in the response to dietary fat.
- To characterize the expression patterns of identified genes.
Main Methods:
- Subtractive hybridization and plus/minus screening of a lambda-cDNA library.
- Construction of cDNA library from mammary gland mRNA of mice on a low-fat diet.
- Differential gene expression analysis using Northern blotting and differential hybridization.
Main Results:
- Isolation of the novel gene LFM-1 (Low Fat Mammary) and stearoyl CoA desaturase.
- LFM-1 showed differential expression in mammary glands, liver, and kidney of mice on low-fat vs. high-fat diets.
- LFM-1 exhibits significant sequence identity to the epsilon subunit of bovine F1-Fo ATPase and a hamster UV-induced gene, suggesting a potential gene family.
Conclusions:
- The developed protocol is effective for identifying diet-regulated genes.
- LFM-1 is a novel gene responsive to dietary fat levels, with specific tissue expression patterns.
- Further investigation into LFM-1 and its gene family may reveal new insights into nutrient metabolism and cellular function.