Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemically modified RNA: approaches and applications

O Heidenreich1, W Pieken, F Eckstein

  • 1Max-Planck-Institut für Experimentelle Medizin, Göttingen, Germany.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of weight loss on the progression of meniscal extrusion and size in knee osteoarthritis: a post-hoc analysis of the Intensive Diet and Exercise for Arthritis (IDEA) trial.

Osteoarthritis and cartilage·2020
Same author

Radiographically normal knees with contralateral joint space narrowing display greater change in cartilage transverse relaxation time than those with normal contralateral knees: a model of early OA? - data from the Osteoarthritis Initiative (OAI).

Osteoarthritis and cartilage·2019
Same author

Cartilage loss in radiographically normal knees depends on radiographic status of the contralateral knee - data from the Osteoarthritis Initiative.

Osteoarthritis and cartilage·2018
Same author

Trajectories of femorotibial cartilage thickness among persons with or at risk of knee osteoarthritis: development of a prediction model to identify progressors.

Osteoarthritis and cartilage·2018
Same author

The role of thigh muscle and adipose tissue in knee osteoarthritis progression in women: data from the Osteoarthritis Initiative.

Osteoarthritis and cartilage·2018
Same author

Is local or central adiposity more strongly associated with incident knee osteoarthritis than the body mass index in men or women?

Osteoarthritis and cartilage·2018

Researchers modified RNA phosphate linkages to phosphorothioates and 2-hydroxyl groups to study essential functional positions. These chemical modifications enhanced ribozyme stability and identified key catalytic groups.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • RNA Therapeutics

Background:

  • Phosphate groups are crucial for RNA structure and function.
  • Modifications of RNA can alter its properties and stability.
  • Ribozymes are catalytic RNA molecules with therapeutic potential.

Purpose of the Study:

  • To investigate the role of phosphate positions in RNA function using phosphorothioate modifications.
  • To explore the impact of 2'-hydroxyl group modifications on ribozyme activity and stability.
  • To identify key hydroxyl groups essential for hammerhead ribozyme catalytic function.

Main Methods:

  • Synthesis of RNA with phosphorothioate internucleotidic linkages.
  • Introduction of modifications at the 2'-hydroxyl position of ribose (e.g., fluorine, amino groups).

Related Experiment Videos

  • Assay of catalytic activity and nuclease stability of modified hammerhead ribozymes.

    • Main Results:

    • Phosphorothioate modifications aided in pinpointing essential phosphate positions.
    • 2'-hydroxyl modifications, including fluorine and amino substitutions, were explored.
    • Key hydroxyl groups critical for hammerhead ribozyme catalysis were identified.
    • Modified ribozymes exhibited increased stability against nucleases.

    Conclusions:

    • Chemical modifications of RNA, specifically phosphorothioates and 2'-hydroxyl substitutions, are valuable tools for functional and structural studies.
    • These modifications enhance ribozyme stability, offering potential for therapeutic applications.
    • The study successfully identified critical residues for hammerhead ribozyme activity.