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Related Experiment Videos

Isolation and characterization of mouse CD7 cDNA

K Yoshikawa1, M Seto, R Ueda

  • 1Department of Urology, Aichi Medical University, Japan.

Immunogenetics
|January 1, 1993
PubMed
Summary

Researchers identified mouse CD7 cDNA, crucial for T-cell development. This discovery aids in understanding T-lymphocyte function and immune system research.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The CD7 antigen is an early T-cell lineage surface marker.
  • Understanding CD7's role is vital for T-lymphocyte research.

Purpose of the Study:

  • To identify and characterize mouse CD7 cDNA.
  • To analyze the structural and functional domains of mouse CD7.

Main Methods:

  • Screening a mouse thymocyte cDNA library using human CD7 cDNA.
  • Northern blot analysis to detect mRNA expression.
  • Hydrophobicity plotting and sequence analysis to predict protein domains.

Main Results:

  • Isolated and sequenced mouse CD7 (mCD7) cDNA, with a 995 bp insert and a 210 amino acid open reading frame.

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  • Detected a 1.2 kb mRNA in thymus, spleen, bone marrow, and small intestine.
  • Deduced protein structure with leader, extracellular, transmembrane, and cytoplasmic domains, showing 45.5% similarity to human CD7.

    • Conclusions:

    • Mouse CD7 cDNA has been successfully identified and characterized.
    • The deduced mCD7 protein shares structural similarities with human CD7.
    • Conserved regions suggest potential roles in T-cell function.