Related Experiment Videos
Inducible T cell antigen 4-1BB. Analysis of expression and function
1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202.
Journal of Immunology (Baltimore, Md. : 1950)
|February 1, 1993
Summary
The 4-1BB protein is expressed on activated T cells and enhances T cell proliferation when stimulated. This suggests 4-1BB acts as an accessory signaling molecule in T cell activation.
Area of Science:
- Immunology
- Molecular Biology
Background:
- 4-1BB is an inducible receptor-like protein found on T cells.
- Its expression is regulated by protein kinase C activation and intracellular calcium levels.
- 4-1BB is classified as an early activation gene.
Purpose of the Study:
- To characterize the expression and function of the 4-1BB molecule.
- To investigate the role of 4-1BB in T cell activation.
Main Methods:
- Generation of a rat monoclonal antibody (mAb) 53A2 against recombinant soluble 4-1BB.
- Flow cytometry (FACS) analysis to detect 4-1BB expression on T cells.
- Cross-linking experiments to assess the effect of 4-1BB stimulation on T cell proliferation.
Main Results:
- Optimal 4-1BB mRNA induction in T cells requires PMA and ionomycin.
- 4-1BB is a 30-kDa glycoprotein, existing as a monomer and a 55-kDa dimer on the cell surface.
- 4-1BB is expressed on activated splenic T cells and thymocytes.
- Cross-linking 4-1BB significantly enhances anti-CD3-stimulated T cell proliferation.
Conclusions:
- 4-1BB is an inducible molecule involved in T cell activation.
- Stimulation of 4-1BB enhances T cell proliferation, indicating its role as an accessory signaling molecule.