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Related Experiment Videos

Cytokine expression from bone marrow derived macrophages

D S Temeles1, H E McGrath, E L Kittler

  • 1University of Virginia Health Sciences Center, Charlottesville 22908.

Experimental Hematology
|February 1, 1993
PubMed
Summary

Murine bone marrow macrophages (BMM) are key producers of CSF-1 and c-kit ligand, and can be induced to express IL-6 and G-CSF. Colony-stimulating factor-1 (CSF-1) differentially regulates BMM cytokine production, potentially controlling stromal hematopoiesis.

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Area of Science:

  • Immunology
  • Hematopoiesis
  • Cell Biology

Background:

  • Monocytes and macrophages exhibit diverse phenotypes based on tissue origin.
  • Peripheral blood monocytes produce various cytokines, but marrow macrophages' cytokine profiles are less understood.
  • Marrow macrophages are a major component of murine Dexter stromal cells and can be isolated using colony-stimulating factor-1 (CSF-1).

Purpose of the Study:

  • To characterize murine bone marrow macrophage (BMM) cytokine production.
  • To investigate the effects of CSF-1, pokeweed mitogen (PWM), and interleukin-3 (IL-3) on BMM cytokine expression.
  • To determine the role of BMM in regulating stromal hematopoiesis.

Main Methods:

  • Murine BMM were cultured with or without CSF-1, PWM, or IL-3.

Related Experiment Videos

  • Conditioned media from BMM cultures were assessed for biologic activity using factor-dependent cell lines.
  • Cytokine presence was confirmed by cell line stimulation, antibody blocking, and mRNA expression analysis (including RIA for CSF-1).

    • Main Results:

    • BMM constitutively express mRNA for CSF-1 and c-kit ligand.
    • BMM can be induced to express interleukin-6 (IL-6) and granulocyte colony-stimulating factor (G-CSF) mRNA.
    • CSF-1 differentially modulates cytokine production, with varying levels affecting IL-6 and c-kit ligand mRNA expression, and higher levels inhibiting CSF-1 mRNA.

    Conclusions:

    • Murine BMM are significant sources of CSF-1 and c-kit ligand.
    • BMM cytokine production, particularly IL-6 and G-CSF, can be induced by stimuli like PWM and IL-3.
    • CSF-1 acts as a central regulator of stromal hematopoiesis by modulating BMM cytokine profiles.