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Phase partitioning detects differences between phospholipase-released forms of alkaline phosphatase--a GPI-linked
F D Raymond1, D W Moss, D Fisher
1Department of Chemical Pathology, Royal Postgraduate Medical School, London, UK.
Biochimica Et Biophysica Acta
|February 13, 1993
Abstract:
A number of enzymes are known to release alkaline phosphatase and other glycan phosphatidylinositol-anchored proteins from membrane surfaces. We describe a novel approach to detect and measure these activities by partitioning in aqueous phase systems. The procedures avoid the complications of micelle-formation involving hydrophobic molecules that may arise with detergent-based partition systems and can clearly distinguish between inositol-specific phospholipase C and D activities.