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Soluble stem cell factor in human serum

K E Langley1, L G Bennett, J Wypych

  • 1Amgen, Inc., Thousand Oaks, CA 91320.

Blood
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

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Stem cell factor (SCF) is crucial for early hematopoiesis. This study quantifies soluble SCF in human serum, revealing its glycosylated nature and functional significance.

Area of Science:

  • Hematology
  • Molecular Biology
  • Biochemistry

Background:

  • Stem cell factor (SCF) plays a vital role in early hematopoiesis.
  • SCF exists in both membrane-bound and soluble forms.
  • Understanding soluble SCF in human serum is important for hematological research.

Purpose of the Study:

  • To determine the levels of soluble stem cell factor (SCF) in human serum.
  • To characterize the nature of SCF present in human serum.
  • To investigate the functional importance of soluble SCF in humans.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to quantify SCF levels in 257 serum samples.
  • Immunoaffinity chromatography was employed for partial purification of serum SCF.

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  • Glycosidase treatments, SDS-PAGE, and immunoblotting were utilized for SCF characterization.

    • Main Results:

    • The average serum SCF level was determined to be 3.3 +/- 1.1 ng/mL.
    • Serum SCF was identified as the soluble form, approximately 165 amino acids long.
    • SCF in serum possesses both N-linked and O-linked glycosylation.

    Conclusions:

    • Soluble stem cell factor (SCF) is present in human serum at quantifiable levels.
    • The characterized soluble SCF is glycosylated and corresponds to the expected molecular size.
    • These findings suggest a significant functional role for soluble SCF in human physiology.