Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear PRP20 protein is required for mRNA export

D C Amberg1, M Fleischmann, I Stagljar

  • 1Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755.

The EMBO Journal
|January 1, 1993
PubMed
Summary

Yeast PRP20 protein, homologous to RCC1, is crucial for mRNA metabolism. Loss of nuclear PRP20 causes poly(A)+ RNA to accumulate in the nucleus, similar to RCC1 defects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemotherapy before or after preoperative chemoradiotherapy and surgery for locally advanced rectal cancer: 5-year results of the CAO/ARO/AIO-12 trial - a general pairwise comparison.

ESMO open·2025
Same author

Inflammatory pathways confer resistance to chemoradiotherapy in anal squamous cell carcinoma.

NPJ precision oncology·2024
Same author

[Radical cystectomy versus trimodality therapy for muscle-invasive bladder cancer].

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2023
Same author

The role of loneliness in the development of depressive symptoms among partnered dementia caregivers: Evidence from the English Longitudinal Study of Aging.

European psychiatry : the journal of the Association of European Psychiatrists·2021
Same author

Posttraumatic stress and youth violence perpetration: A population-based cross-sectional study.

European psychiatry : the journal of the Association of European Psychiatrists·2016
Same author

Facile synthesis of one-dimensional organometallic-organic hybrid polymers based on a diphosphorus complex and flexible bipyridyl linkers.

Chemical communications (Cambridge, England)·2016

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The yeast PRP20 protein shares significant structural and functional homology with the RCC1 protein found in higher eukaryotes.
  • RCC1 protein regulates the initiation of mitosis, while PRP20 is essential for precise and efficient mRNA metabolism.
  • Mutant prp20 cells exhibit a loss of nuclear PRP20 protein upon temperature shift, leading to observable phenotypes.

Purpose of the Study:

  • To investigate the function of the yeast PRP20 protein in mRNA metabolism.
  • To compare the cellular phenotypes associated with defects in yeast PRP20 and mammalian RCC1 proteins.
  • To understand the role of PRP20 in nuclear RNA localization.

Main Methods:

  • Analysis of mutant yeast strains (prp20) shifted to non-permissive temperatures.

Related Experiment Videos

  • Observation of poly(A)+ RNA localization using microscopy.
  • Comparison with existing data from temperature-sensitive hamster cell lines (tsBN2) with RCC1 mutations.

    • Main Results:

    • A temperature-sensitive mutation in yeast prp20 leads to the rapid loss of nuclear PRP20 protein.
    • This loss is accompanied by the accumulation of polyadenylated RNA (poly(A)+ RNA) within the nucleus.
    • Similar nuclear mRNA accumulation is observed in tsBN2 hamster cells carrying a temperature-sensitive RCC1 allele.

    Conclusions:

    • The yeast PRP20 protein plays a critical role in maintaining proper mRNA metabolism and localization within the nucleus.
    • PRP20 function is analogous to that of RCC1 in higher eukaryotes concerning nuclear RNA regulation.
    • Disruptions in PRP20 or RCC1 function result in a conserved phenotype of nuclear mRNA accumulation.