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Egg viability in urinary schistosomiasis. I. New methods compared with available methods

R A Braun-Munzinger1, B A Southgate

  • 1Project Bilharziose, Ministère de la Santé de la République Démocratique de Madagascar, Mahajanga.

The Journal of Tropical Medicine and Hygiene
|February 1, 1993
PubMed
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Egg viability in urinary schistosomiasis. III. Repeatability and reproducibility of new methods.

The Journal of tropical medicine and hygiene·1993

A novel method accurately quantifies Schistosoma haematobium egg viability and hatchability in urine. This simple, cost-effective technique is ideal for large-scale field studies.

Area of Science:

  • Parasitology
  • Clinical Diagnostics
  • Public Health

Background:

  • Schistosomiasis diagnosis relies on detecting Schistosoma haematobium eggs in urine.
  • Accurate quantification of egg viability and hatchability is crucial for assessing transmission potential and treatment efficacy.
  • Existing methods for egg viability assessment have limitations, including sample loss and unclassifiable results.

Purpose of the Study:

  • To develop and evaluate a new, simple, and cost-effective method for quantifying Schistosoma haematobium egg viability and hatchability in urine samples.
  • To compare the performance of the new method against established techniques: centrifugation hatching and Nuclepore filtration with trypan blue staining.

Main Methods:

  • Urine samples were filtered using Nytrel filters.

Related Experiment Videos

  • Viable (motile) miracidia within egg shells were detected using mercurochrome staining.
  • Hatched miracidia were identified using iodine staining.
  • Comparison with centrifugation hatching and Nuclepore filtration trypan blue staining methods using 73 egg-positive urine samples.

    • Main Results:

    • The new method showed close agreement with centrifugation hatching for overall viable egg proportions.
    • Centrifugation hatching resulted in a 33% loss of eggs.
    • Nuclepore filtration with trypan blue staining left 19% of eggs unclassifiable.
    • The new method demonstrated high viability and hatchability quantification accuracy.

    Conclusions:

    • The developed Nytrel filtration and dual staining method offers a reliable alternative for assessing Schistosoma haematobium egg viability and hatchability.
    • Its simplicity, low cost, and ease of performance make it highly suitable for large-scale field applications.
    • Further standardization is recommended to optimize its widespread use in schistosomiasis control programs.