Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epitope-tagging vectors designed for yeast

P Reisdorf1, A C Maarse, B Daignan-Fornier

  • 1Laboratoire de Génétique Moléculaire, Université Paris-Sud, Orsay, France.

Current Genetics
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yeast AMP pathway genes respond to adenine through regulated synthesis of a metabolic intermediate.

Molecular and cellular biology·2001
Same author

Proteome analysis and morphological studies reveal multiple effects of the immunosuppressive drug mycophenolic acid specifically resulting from guanylic nucleotide depletion.

The Journal of biological chemistry·2001
Same author

YLR209c encodes Saccharomyces cerevisiae purine nucleoside phosphorylase.

Journal of bacteriology·2001
Same author

Alteration of transforming growth factor-beta1 response involves down-regulation of Smad3 signaling in myofibroblasts from skin fibrosis.

The American journal of pathology·2001
Same author

Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes.

Yeast (Chichester, England)·2001
Same author

Highly conserved features of DNA binding between two divergent members of the Myb family of transcription factors.

Nucleic acids research·2001
Same journal

Is heptelidic (koningic) acid a microbial hormone that regulates secondary metabolism in the biocontrol fungus Trichoderma virens?

Current genetics·2025
Same journal

Loss of PDR3 alters metabolome in response to MCHM, a synthetic hydrotrope.

Current genetics·2025
Same journal

The CRISPR-cas repertoire of Kluyvera ascorbata: insights from genomic data.

Current genetics·2025
Same journal

Genome characterization of Acinetobacter species from the rice rhizosphere: a potential plant growth promoting rhizobacteria (PGPR).

Current genetics·2025
Same journal

Genomic surveillance of vancomycin-resistant Enterococcus faecium: a study on Resistome, Plasmidome, and mobilome profiling.

Current genetics·2025
Same journal

Epigenetic regulation of pathogenicity in fungi.

Current genetics·2025
See all related articles

Researchers developed new yeast vectors for easy epitope tagging of proteins using a human c-myc tag. This simplifies the process of studying yeast protein function and interactions.

Area of Science:

  • Molecular Biology
  • Yeast Genetics

Background:

  • Epitope tagging is crucial for protein analysis in yeast.
  • Existing methods for epitope tagging can be complex and time-consuming.

Purpose of the Study:

  • To create novel Saccharomyces cerevisiae-Escherichia coli shuttle vectors for simplified epitope tagging.
  • To facilitate the fusion of a human c-myc epitope tag to the 3' end of yeast genes.

Main Methods:

  • Construction of two shuttle vectors designed for yeast and E. coli.
  • Inclusion of a sequence encoding the human c-myc epitope.

Main Results:

  • Successfully developed vectors enabling 3' end gene fusion with the c-myc epitope.
  • Demonstrated the utility of the technique with an example application.

Related Experiment Videos

Conclusions:

  • The new vectors streamline the epitope-tagging process in yeast.
  • This facilitates downstream applications such as protein detection and purification.