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Related Experiment Videos

Basophil mediator release in atopic dermatitis

H A Bull1, P F Courtney, C B Bunker

  • 1Department of Dermatology, University College and Middlesex School of Medicine, Middlesex Hospital, London, U.K.

The Journal of Investigative Dermatology
|March 1, 1993
PubMed
Summary
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Basophils from adults with atopic dermatitis release less leukotriene C4 than unaffected individuals. H3 receptors do not influence histamine release from basophils in this condition.

Area of Science:

  • Immunology
  • Dermatology

Background:

  • Basophils are implicated in atopic dermatitis via histamine and eicosanoid release.
  • Mechanisms regulating basophil mediator release remain unclear.
  • H3 receptors control histamine synthesis and release in nervous tissue.

Purpose of the Study:

  • Investigate histamine, leukotriene C4 (LTC4), and prostaglandin D2 release from anti-immunoglobulin E (IgE)-stimulated basophils in atopic dermatitis patients and controls.
  • Determine the role of H3 receptors in basophil mediator release using agonists and antagonists.

Main Methods:

  • Prepared basophil-rich leukocyte fractions from adult atopic dermatitis patients and unaffected individuals.
  • Stimulated basophils with anti-IgE to measure histamine, LTC4, and prostaglandin D2 release.
  • Co-incubated basophils with anti-IgE and H3 receptor agonist or antagonist.

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Main Results:

  • Anti-IgE induced concentration-dependent release of histamine and LTC4, but not prostaglandin D2.
  • Histamine release levels were similar between atopic and normal basophils.
  • Anti-IgE-stimulated LTC4 release was significantly lower in atopic basophils compared to normal basophils.
  • Neither H3 receptor agonist nor antagonist modulated anti-IgE-induced histamine release.

Conclusions:

  • Adult atopic dermatitis basophils release comparable histamine but less LTC4 than those from unaffected adults.
  • H3 receptors are not involved in anti-IgE-induced histamine release from human basophils.
  • These findings do not support a role for increased basophil histamine release in adult atopic dermatitis symptoms.