Nucleotide sequence of the large mitochondrial rRNA gene of Penicillium chrysogenum

The nucleotide sequence of a large rRNA (1-rRNA) gene and its flanking regions in the cloned fragments of mitochondrial (mt) DNA from Penicillium chrysogenum NRRL1951 (Sekiguchi J., Ohsaki, T., Yamamoto, H., Koichi, K. and Shida, T. (1990) J. Gen. Microbiol. 136, 535-543) was determined and compared with those in Aspergillus nidulans and Neurospora crassa mitochondrial DNAs. The P. chrysogenum mt 1-rRNA gene has a 1678 bp intron which intervenes between a 2835 bp 5' exon and a 581 bp 3' exon, and extensive homology exists between overall sequences of mt 1-rRNA genes of P. chrysogenum and A. nidulans. The P. chrysogenum intron contains a large open reading frame which encodes a polypeptide comprising of 399 amino acids. The intron sequence also suggests that the intron belongs to a self-splicing group IA.