Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Staining with fluorescein diacetate correlates with hepatocyte function

S L Nyberg1, R A Shatford, W D Payne

  • 1Department of Surgery, University of Minnesota, Minneapolis 55455.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Voronoi theory of the normal liver lobular architecture and its applicability in hepatic zonation.

Scientific reports·2021
Same author

Impact of molecular adsorbent recirculating system therapy on tacrolimus elimination: a case report.

Transplantation proceedings·2014
Same author

Myth-conceptions about death.

Journal of religion and health·2014
Same author

Prospective randomized trial of maintenance immunosuppression with rapid discontinuation of prednisone in adult kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2013
Same author

Development of a bioartificial liver employing xenogeneic hepatocytes.

Cytotechnology·2012
Same author

Development of a bioartificial liver device.

Methods in molecular medicine·2011

Fluorescein diacetate (FDA) staining accurately assesses cultured hepatocyte viability and function. High FDA fluorescence strongly correlates with increased albumin production and P-450 activity, validating its use as a reliable viability marker.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Toxicology

Background:

  • Hepatocyte viability is crucial for drug metabolism and toxicity studies.
  • Accurate assessment of cell viability is essential for reliable experimental outcomes.
  • Fluorescein diacetate (FDA) is a potential stain for evaluating cell health.

Purpose of the Study:

  • To validate fluorescein diacetate (FDA) as a reliable stain for assessing cultured hepatocyte viability.
  • To determine the correlation between FDA staining intensity and hepatocyte function.

Main Methods:

  • Cultured hepatocytes were stained with FDA and analyzed using flow cytometry.
  • Live and dead cell mixtures were used to establish correlation with viability.
  • Hepatocytes were sorted based on fluorescence intensity for functional assays.

Related Experiment Videos

Main Results:

  • FDA staining showed a strong positive correlation (R2 = 0.962) with hepatocyte viability.
  • High FDA fluorescence group exhibited significantly increased albumin production.
  • High FDA fluorescence group demonstrated elevated lidocaine metabolism (P-450 activity).

Conclusions:

  • FDA staining is a valuable and rapid method for assessing cultured hepatocyte viability.
  • FDA fluorescence intensity serves as a reliable indicator of key hepatocyte functions.
  • This assay supports accurate evaluation in drug discovery and toxicology research.