Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endotoxin-neutralizing capacity of soluble CD14 is a highly conserved specific function

U Grunwald1, C Krüger, C Schütt

  • 1Department of Medical Immunology, Ernst-Moritz-Arndt-University of Greifswald, Germany.

Circulatory Shock
|March 1, 1993
PubMed
Summary

Soluble CD14 (sCD14) protects cells from endotoxin activation by blocking lipopolysaccharide (LPS) binding. This conserved mechanism is vital for preventing septic shock.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inflammatory pseudo-tumour of the colon mimicking acute appendicitis: A case report.

International journal of surgery case reports·2022
Same author

[Terbinafine-resistant dermatophytoses and onychomycosis due to Trichophyton rubrum].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2021
Same author

Activity of nadifloxacin and three other antimicrobial agents against Cutibacterium acnes isolated from patients with acne vulgaris.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

[Toxic shock syndrome due to <i>Staphylococcus aureus</i> in a small child, a (clinical or laboratory chemical) visual diagnosis?]

Monatsschrift Kinderheilkunde : Organ der Deutschen Gesellschaft fur Kinderheilkunde·2020
Same author

What is the outcome of re-recurrent vs recurrent inguinal hernia repairs? An analysis of 16,206 patients from the Herniamed Registry.

Hernia : the journal of hernias and abdominal wall surgery·2020
Same author

Measurement of the <math></math> energy spectrum with IceCube-79: IceCube Collaboration.

The European physical journal. C, Particles and fields·2020

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Human monocytes bind lipopolysaccharides (LPS) via the CD14 glycoprotein, a process facilitated by LPS-binding protein (LBP).
  • Soluble CD14 (sCD14) is a serum protein involved in modulating LPS-monocyte interactions.

Purpose of the Study:

  • To investigate the role of soluble CD14 (sCD14) in neutralizing endotoxin activity.
  • To determine the species specificity and conserved function of sCD14 in LPS binding and cell activation.

Main Methods:

  • Flow cytometry was employed to quantify the binding of fluorescein isothiocyanate (FITC)-labeled LPS to monocytes.
  • Monoclonal antibodies (mAbs) were used to block specific interactions between LBP, LPS, and CD14.

Main Results:

Related Experiment Videos

  • Bovine monocytes showed LPS-FITC binding mediated by calf serum, which was abrogated by human sCD14.
  • The inhibitory function of sCD14 was specifically blocked by an antibody (MEM-18) targeting the LBP-LPS binding site on CD14.
  • Monoclonal antibodies against human CD14 confirmed the species-specific blocking of sCD14 function, highlighting conserved mechanisms.

Conclusions:

  • Soluble CD14 (sCD14) possesses a critical biological function in protecting cells from endotoxin-induced activation.
  • The LBP-LPS binding region of CD14 is a highly conserved structure across species, suggesting a universal role in endotoxin neutralization.
  • These findings have implications for understanding and treating pathological conditions like septic shock.