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The effects of oral RNA and intraperitoneal nucleoside-nucleotide administration on methicillin-resistant
A A Adjei1, F Takamine, H Yokoyama
1Department of Nutrition, University of the Ryukyus, Okinawa, Japan.
Abstract:
The effects of oral RNA and intraperitoneal nucleoside-nucleotide mixture administration on methicillin-resistant Staphylococcus aureus (MRSA) strain 8985N infection were studied in mice. BALB/c mice were fed a nucleic acid-free diet or nucleic acid-free diet supplemented with 0.5% or 2.5% ribonucleic acid (RNA) for 30 days. Nucleoside-nucleotide mixture or saline (control) was intraperitoneally administered daily to these rats except for the 2.5% RNA group, which received saline only. On the 10th day of this treatment, the mice were inoculated intravenously with the viable MRSA organisms. Susceptibility to the MRSA was determined by animal survival and recovery of the MRSA from the organs. The survival rates in the three groups that were administered saline were 29%, 35%, and 40% for nucleic acid-free diet, 0.5% RNA, and 2.5% RNA groups, respectively, whereas in the two groups that received the nucleoside-nucleotide mixture the rates were 69% for the nucleic acid-free diet group and 55% for 0.5% RNA group. The susceptibility of the mice to the MRSA challenge was not affected by dietary RNA, which indicates the ineffectiveness of oral RNA. The combined survival rate in the two nucleoside-nucleotide groups (64%) was statistically different (p < .01) from that in the three saline groups (34%). There was a greater reduction in viable organism recovery in the kidney and spleen of the surviving mice that had been administered the nucleoside-nucleotide mixture than in those administered saline.(ABSTRACT TRUNCATED AT 250 WORDS)
Insights
Intraperitoneal administration of a nucleoside-nucleotide mixture significantly improved survival rates in mice challenged with methicillin-resistant Staphylococcus aureus (MRSA). Oral ribonucleic acid (RNA) did not enhance resistance to MRSA infection.
Area of Science:
- Immunology
- Microbiology
- Pharmacology
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat.
- Understanding host-pathogen interactions and potential therapeutic interventions is crucial for combating MRSA infections.
Purpose of the Study:
- To evaluate the efficacy of oral ribonucleic acid (RNA) and intraperitoneal nucleoside-nucleotide mixture administration against MRSA infection in a murine model.
- To determine if dietary RNA or nucleoside-nucleotide supplementation impacts host susceptibility to MRSA.
Main Methods:
- BALB/c mice were fed diets with or without RNA supplementation for 30 days.
- Mice received daily intraperitoneal injections of a nucleoside-nucleotide mixture or saline.
- Mice were intravenously inoculated with MRSA strain 8985N.
- Survival rates and MRSA recovery from organs were assessed.
Main Results:
- Survival rates were 29-40% in saline-treated groups and 55-69% in nucleoside-nucleotide mixture-treated groups.
- The nucleoside-nucleotide mixture significantly increased survival (64%) compared to saline (34%) (p < .01).
- Dietary RNA supplementation did not affect survival rates, indicating its ineffectiveness against MRSA.
Conclusions:
- Intraperitoneal administration of a nucleoside-nucleotide mixture enhances host resistance to MRSA infection.
- Oral RNA supplementation is ineffective in improving susceptibility to MRSA.
- Nucleoside-nucleotide mixtures show potential as an adjunctive therapy for MRSA infections.