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Related Experiment Videos

Leukocytes synthesize angiotensinogen

R A Gomez1, L L Norling, N Wilfong

  • 1Department of Pediatrics, University of Virginia Medical School, Charlottesville.

Hypertension (Dallas, Tex. : 1979)
|April 1, 1993
PubMed
Summary

Circulating leukocytes express the angiotensinogen gene, synthesizing and releasing angiotensinogen. This finding suggests leukocytes may act as a mobile system for generating angiotensin, impacting inflammatory responses and hypertension.

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Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • Leukocytes play a crucial role in immune responses and inflammation.
  • The renin-angiotensin system (RAS) is vital for blood pressure regulation.
  • The cellular sources of angiotensinogen, particularly in non-hepatic tissues, are not fully elucidated.

Purpose of the Study:

  • To investigate the expression of the angiotensinogen gene in leukocytes.
  • To determine if leukocytes synthesize and release functional angiotensinogen.
  • To explore the potential role of leukocyte-derived angiotensinogen in local physiological processes.

Main Methods:

  • Northern blot analysis to detect angiotensinogen messenger RNA (mRNA) in rat leukocytes and murine bone marrow cells.
  • Western blot analysis to identify angiotensinogen protein isoforms in rat leukocyte homogenates.

Related Experiment Videos

  • Metabolic labeling and immunoprecipitation to confirm angiotensinogen synthesis and release by rat leukocytes.
  • Enzymatic assay using hog renin to assess the generation of angiotensin I from leukocyte-conditioned media.

    • Main Results:

    • Angiotensinogen mRNA sequences were detected in circulating rat leukocytes and murine bone marrow stromal cells.
    • Rat leukocytes possess two main angiotensinogen isoforms (46.5 and 53.9 kd).
    • Rat leukocytes synthesize and release angiotensinogen protein, which can be cleaved by renin to generate angiotensin I.

    Conclusions:

    • Circulating leukocytes express the angiotensinogen gene, producing and secreting angiotensinogen.
    • Leukocytes represent a mobile angiotensin-generating system.
    • Leukocyte-derived angiotensin may influence local inflammatory responses, tissue injury, and arterial hypertension.