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Intercellular adhesion molecules in normal synovium

K Fairburn1, M Kunaver, L S Wilkinson

  • 1Department of Rheumatology Research, University College and Middlesex School of Medicine, London.

British Journal of Rheumatology
|April 1, 1993
PubMed
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Researchers investigated intercellular adhesion molecules in normal synovium and skin vasculature. E-selectin and ICAM-1 were detected in synovial vessels, comparable to skin, suggesting localized expression rather than systemic inflammation.

Area of Science:

  • Immunology
  • Vascular Biology
  • Histology

Background:

  • Intercellular adhesion molecules (ICAMs) are crucial for cell-cell interactions.
  • Cytokines upregulate ICAM expression on endothelial cells in vitro.
  • Understanding ICAM expression in normal tissues provides a baseline for disease states.

Purpose of the Study:

  • To examine the presence and distribution of E-selectin, ICAM-1, and VCAM-1 in normal synovial and dermal vasculature.
  • To compare ICAM expression levels between synovial and skin tissues.
  • To assess the potential role of cytokines in regulating ICAM expression in vivo.

Main Methods:

  • Immunohistochemistry and microdensitometry were used to analyze cryostat sections of normal synovium and skin.
  • Specific monoclonal antibodies (1.2B6 for E-selectin, 6.5B5 for ICAM-1, 1.4C3 for VCAM-1) were employed.

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  • Expression levels were quantified using mean absorption indices.

    • Main Results:

    • E-selectin and ICAM-1 were detected in normal synovial venules at levels similar to or lower than dermal vessels.
    • E-selectin expression was highest on superficial synovial venules; ICAM-1 was most prominent on deep synovial venules.
    • VCAM-1 showed low expression on vessel-associated cells, with no significant endothelial staining, but was present in the synovial lining layer.

    Conclusions:

    • Normal synovial vasculature expresses E-selectin and ICAM-1, with distinct patterns of distribution.
    • The expression levels are comparable to those in normal skin, suggesting localized regulation.
    • VCAM-1 is minimally expressed on endothelial cells in these normal tissues.