Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA digest analysis with capillary electrophoresis

A Paulus1, D Hüsken

  • 1Corporate Analytical Research, Ciba-Geigy Ltd., Basal, Switzerland.

Electrophoresis
|January 1, 1993
PubMed
Summary

Capillary electrophoresis using polymer-filled capillaries offers enhanced DNA fragment separation for small fragments up to 600 base pairs. This automated method provides faster analysis and better resolution compared to traditional slab gel electrophoresis in this range.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maternal separation differentially modulates early pathology by sex in 5xFAD Alzheimer's disease-transgenic mice.

Brain, behavior, & immunity - health·2023
Same author

Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacterium.

The ISME journal·2022
Same author

The tensile properties of poly(ethylene oxide)-based segmented block copolymers in the dry and wet state.

Journal of materials science·2022
Same author

Early-life stress elicits peripheral and brain immune activation differently in wild type and 5xFAD mice in a sex-specific manner.

Journal of neuroinflammation·2022
Same author

Maternal separation leads to regional hippocampal microglial activation and alters the behavior in the adolescence in a sex-specific manner.

Brain, behavior, & immunity - health·2021
Same author

[Neuroendocrine neoplasms : a new era to the top of multidisciplinarity !]

Revue medicale de Liege·2021

Area of Science:

  • Molecular Biology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • DNA digest analysis is crucial for molecular biology applications.
  • Traditional slab gel electrophoresis has limitations in resolution and speed.

Purpose of the Study:

  • To evaluate the effectiveness of polymer-filled capillaries in CE for DNA digest analysis.
  • To compare the resolution and efficiency of CE with traditional slab gel electrophoresis.
  • To explore the benefits of CE for automated DNA fragment analysis.

Main Methods:

  • DNA samples (100 base pair ladder up to 2000 base pairs) were analyzed.
  • Separations were performed using automated capillary electrophoresis with 6% polyacrylamide-filled capillaries.

Related Experiment Videos

  • Capillary inner walls were coated with methacryloxysilane for polyacrylamide binding.
  • Comparison with traditional slab gel electrophoresis at various agarose concentrations.

    • Main Results:

    • Excellent separation of DNA fragments up to 600 base pairs was achieved with CE, resolving fragments differing by less than 10 base pairs.
    • Resolution decreased for fragments between 600 and 2200 base pairs.
    • CE demonstrated superior resolution for small DNA fragments (<600 bp) compared to slab gels.
    • Slab gels were superior for fragments above 600 base pairs with the tested CE column composition.

    Conclusions:

    • Polymer-filled capillary electrophoresis provides high-resolution separation for small DNA fragments.
    • CE offers advantages in speed, automation, and sample loading capacity.
    • CE is a valuable alternative to slab gel electrophoresis for specific DNA analysis applications.