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Circularly permuted DNA, RNA and proteins--a review
1Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Gene
|March 30, 1993
Summary
Circular permutation alters macromolecular folding and function. A new method analyzes RNA folding for all circularly permuted isomers, demonstrated with transfer RNA and a viral protein binding site.
Area of Science:
- Biochemistry
- Molecular Biology
- Structural Biology
Background:
- Circular permutation is a type of macromolecular isomerization.
- It involves linking normal termini and introducing new ones by backbone cleavage.
- Understanding its impact on folding and function is crucial for biological macromolecules.
Purpose of the Study:
- To describe the implications of circular permutation on macromolecular folding and function.
- To present a novel method for analyzing the folding of all circularly permuted RNA isomers.
Main Methods:
- Development of a method to analyze the folding of circularly permuted RNA isomers.
- Application of the method to a transfer RNA (tRNA).
- Application of the method to the bacteriophage R17 coat protein binding site.
Main Results:
- The study details the effects of circular permutation on macromolecular folding and function.
- A new analytical method for circularly permuted RNA isomers was successfully developed and applied.
- The method was validated using a tRNA and the R17 coat protein binding site.
Conclusions:
- Circular permutation significantly impacts the folding and function of biological macromolecules.
- The presented method provides a powerful tool for analyzing the folding of various circularly permuted RNA structures.
- This work advances the understanding of RNA structure-function relationships in both cellular and viral contexts.