Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chronic lymphocytic leukemia with low lymphocyte count

A Batata1, B Shen

  • 1Department of Pathology, Cox Institute, Wright State University School of Medicine, Dayton, Ohio 45429.

Cancer
|May 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Safety and efficacy of electrical impedance tomography-guided airway clearance in mechanically ventilated patients].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2026
Same author

Consensus statement on the use of systemic therapies in pancreatic ductal adenocarcinoma in Asia.

ESMO gastrointestinal oncology·2026
Same author

[Study on the mediating effect of fatigue on neck WMSDs in the footwear industry].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Ergonomic evaluation and effectiveness analysis of improvement measures in the assembly position of an automotive parts production company].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
Same author

[Anticoagulation effects of nafamostat mesylate in sustained low-efficiency dialysis and its relevant factors].

Zhonghua yi xue za zhi·2025
Same author

Corrigendum to "Safety and efficacy of odronextamab in patients with relapsed or refractory follicular lymphoma": [Ann Oncol 35 (2024) 1039-1047].

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Chronic lymphocytic leukemia with low absolute lymphocyte count (CLL-LLC) presents similar immunologic markers to typical CLL. Early immunophenotyping is crucial for diagnosing CLL-LLC, which constitutes 6% of B-CLL cases.

Area of Science:

  • Hematology
  • Oncology
  • Immunology

Background:

  • Diagnosis of chronic lymphocytic leukemia (CLL) typically requires an absolute lymphocyte count (ALC) > 5 x 10^9/l.
  • Cases with low ALC (CLL-LLC) are often overlooked and not included in current staging systems.
  • The diagnostic significance of differential lymphocyte counts in CLL-LLC remains underemphasized.

Purpose of the Study:

  • To investigate the immunologic characteristics of CLL-LLC.
  • To compare CLL-LLC with typical CLL using immunophenotyping and cytochemical analysis.
  • To highlight the importance of immunologic investigations for diagnosing CLL-LLC.

Main Methods:

  • Peripheral blood samples from 12 CLL-LLC and 189 typical CLL patients were analyzed.
  • Immunologic evaluation included surface immunoglobulin (SIg), mouse erythrocyte rosettes, CD5, CD19, CD20, CD22, and CD2.

Related Experiment Videos

  • Cytochemical evaluation of tartrate-resistant acid phosphatase (TRAP) was performed.

    • Main Results:

    • CLL-LLC patients (ages 47-84) had ALC from 1.5-4.9 x 10^9/l and 52-93% lymphocytes.
    • CLL-LLC exhibited immunophenotypic similarity to typical CLL, including weak monoclonal SIg, positive CD5, and high CD19/CD20 expression.
    • No statistical differences were found in immunologic markers or SIg isotype distribution between CLL-LLC and typical CLL.

    Conclusions:

    • CLL-LLC accounts for 6% of B-cell CLL and may be missed without immunologic investigation due to low ALC and absence of symptoms.
    • Persistent relative lymphocytosis (≥50%) in individuals over 50 warrants further investigation for CLL via immunophenotyping.
    • Immunophenotyping of peripheral blood lymphocytes and bone marrow examination are essential for diagnosing CLL-LLC.