Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

27.0K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.0K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

32.7K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.7K
Fixed Action Patterns01:06

Fixed Action Patterns

17.6K
A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
17.6K
RNA Interference01:23

RNA Interference

28.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.0K
RNA Structure01:23

RNA Structure

79.0K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.0K
RNA Stability01:53

RNA Stability

35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimization of a Genome-Wide Disordered Lentivector-Based Short Hairpin RNA Library.

Molecular biology·2011
Same author

[Genome-wide lentivector-based pooled shRNA library optimization].

Molekuliarnaia biologiia·2006
Same author

Modified dinucleoside tetraphosphonates, new potential inhibitors of HIV reverse transcriptase.

Nucleosides, nucleotides & nucleic acids·2001
Same author

Modified nucleoside 5'-triphosphonates as a new type of antiviral agents.

Nucleosides & nucleotides·1999
Same author

[A method for obtaining the normalized cDNA libraries based on the effect of suppression of polymerase chain reaction].

Bioorganicheskaia khimiia·1996
Same author

Combining the technique of RNA fingerprinting and differential display to obtain differentially expressed mRNA.

Biochemical and biophysical research communications·1996

Related Experiment Video

Updated: Jan 30, 2026

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
06:22

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Published on: October 27, 2020

4.7K

Analysis of poly(A)+RNA patterns in human tissues

A A Chenchik1, L B Diachenko, R S Beabealashvilli

  • 1Cardiology Research Center, Russian Academy of Medical Sciences, Moscow.

FEBS Letters
|April 19, 1993
PubMed
Summary

This study introduces a new method to analyze messenger RNA (mRNA) in biological samples. The technique reveals distinct mRNA patterns in human tissues and identifies differences between normal and tumor cells.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Analyzing messenger RNA (mRNA) abundance is crucial for understanding gene expression.
  • Existing methods for comparing mRNA profiles across different samples can be complex and labor-intensive.

Purpose of the Study:

  • To develop a novel, efficient method for analyzing and comparing relative mRNA amounts in polyadenylated RNA (poly(A)+RNA) preparations.
  • To identify differences in mRNA expression patterns between various human tissues and between normal and neoplastic tissues.

Main Methods:

  • The method involves synthesizing short complementary DNA (cDNA) termination products using reverse transcription.
  • Poly(A)+RNA is primed with a set of 30 unique 5'-labeled oligonucleotides, ensuring broad coverage of individual RNAs.

More Related Videos

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

6.7K
Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
06:44

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Published on: January 26, 2019

8.4K

Related Experiment Videos

Last Updated: Jan 30, 2026

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
06:22

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Published on: October 27, 2020

4.7K
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

6.7K
Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
06:44

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Published on: January 26, 2019

8.4K
  • Products are analyzed via denaturing polyacrylamide gel electrophoresis, generating distinct banding patterns.

    • Main Results:

    • The technique effectively analyzes and compares the relative abundance of abundant individual mRNAs ( > 0.1% abundance).
    • Distinct poly(A)+RNA patterns were observed across different human tissues.
    • Significant changes in RNA patterns were identified between normal human tissues and neoplastic tissues, specifically uterine myoma.

    Conclusions:

    • The developed method provides a robust approach for comparative mRNA profiling.
    • This technique is valuable for identifying tissue-specific gene expression and molecular differences in diseases like cancer.
    • The findings highlight the utility of this method in biomedical research and diagnostics.