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Effect of downstream message secondary structure on the secretory expression of the Streptomyces subtilisin inhibitor
S Taguchi1, Y Yoshida, I Kumagai
1Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan.
FEMS Microbiology Letters
|March 1, 1993
Abstract:
The contribution of downstream message secondary structure to the production of Streptomyces subtilisin inhibitor (SSI) was investigated in Streptomyces lividans 66 using a cloned gene. By deletion analysis, two inverted repeats located downstream from the stop codon in the SSI gene were found to exhibit a marked effect on the amount and homogeneity of SSI gene transcript and, consequently, the efficiency of SSI productivity.